Amido black 10B | |
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4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt |
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Other names
Amidoschwarz |
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Identifiers | |
CAS number | 1064-48-8 |
PubChem | 9566044 |
ChemSpider | 10660833 |
Jmol-3D images | Image 1 |
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Properties | |
Molecular formula | C22H14N6Na2O9S2 |
Molar mass | 616.49 g mol−1 |
Appearance | Dark red to black |
Solubility in water | ~ 30 g/l at 20 °C |
Hazards | |
R-phrases | R20, R21, R33 |
S-phrases | S28A, S37, S45 |
NFPA 704 |
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(verify) (what is: / ?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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Infobox references |
Amido black 10B is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.